The aide, media adviser Ali Akbar Javanfekr, was also sentenced to a year in jail and banned from journalism over a separate publication which was deemed to have offended public decency, the semi-official Fars news agency reported.
Both incidents spotlighted a feud between Ahmadinejad's camp and others in the conservative establishment that runs the world's fifth biggest oil exporter and faces increasing international pressure over its nuclear activities.
Tehran's prosecutor's office ordered the daily Etemad to close for two months for "disseminating lies and insults to officials in the establishment," according to Fars.
In the interview in Saturday's edition, Javanfekr hit back at critics who accuse Ahmadinejad of being in the thrall of a "deviant" circle seeking to undermine the Islamic establishment, saying they had "poisoned" politics and implying many were corrupt.
"What have we 'deviated' from? Yes, we have deviated from those friends, from their beliefs, behavior and interpretations," Javanfekr, who also heads the official Iranian news agency IRNA, told Saturday's Etemad.
"If they meant the deviant current is a deviation from their beliefs, we confirm it."
The counterattack, published verbatim over three pages, signaled the determination of Ahmadinejad's camp to fight back as Iran gears up for parliamentary elections in March.
Javanfekr's lawyer told Reuters he had not been notified of the jail sentence and three-year ban from journalism imposed by the prosecutor's office following a guilty verdict pronounced by the Press Supervisory Board earlier this month.
Abdollah Nakhaie said he would appeal the sentence which, according to the ISNA news agency, he has 20 days to do.
