The burning of an American flag during a protest this week was an isolated incident, but one which captured the mood.
"People are getting more frustrated. Frustration always breeds anger," said one Palestinian official who asked not to be named, giving a private assessment of public feeling.
Recently, signs advertising US financial support for Palestinian West Bank development projects have been vandalized, one sprayed with the word "Veto" — the fate the Obama administration has in store for the Palestinian UN bid.
Regardless of American warnings, Palestinian President Mahmoud Abbas plans to submit the application for recognition of Palestinian statehood and full UN membership on Friday at the UN General Assembly in New York.
More than 1,000 Palestinians carrying signs denouncing Obama gathered outside the West Bank headquarters of Palestinian President Mahmoud Abbas before marching into the city center shouting: "It's shameful for America to support the occupation."
"America is the head of the snake," bellowed demonstrators in Ramallah as they held up signs deriding Obama for his address, in which he warned the Palestinians there would be "no shortcuts" to peace.
"It was totally disappointing, not only to us but to many people in this world. It was full of double standards," said Mustafa Barghouti, a Palestinian politician and activist who ran for the presidency in 2005.
"In reality, when we need them, we find that they are the ones who most conspire against the state of Palestine, against the destiny of the Palestinian people," said Nazih Qabaha, 30.
"We no longer have any faith in US policy. Regardless of who the president is, it is the same policy," added Qabaha, an employee of the Palestinian Authority.
In Gaza City, around 300 women held a protest outside the UN headquarters, shouting anti-Obama slogans as Hamas security forces watched them.
The Palestinian workers union said it would call for rallies outside US embassies across the Arab world on Friday.
