The victory was the third in a row on the American indoor circuit for the 24-year-old, who had never previously made a US international team at the senior level.
"I never would have thought I would have run 6.49, but it happened," said the 2004 world junior champion who stamped himself as a major contender for the sprint title at the March 12-14 IAAF world indoor championships in Doha, Qatar.
His time, a personal best, was one hundredth of a second faster than the previous best time for the year set by Briton Dwain Chambers.
Former US indoor champion Mike Rodgers was second in 6.52 seconds. The top two finishers in each event make the American world championships team if they have met qualifying standards.
Carmelita Jeter dominated the women's 60, running 7.02 seconds, the second fastest time in the world this season.
Two-time world indoor hurdles champion Terrence Trammell, outdoor women's long jump world gold medallist Brittney Reese and the surprising Debbie Dunn also set world seasonal bests at the trials, which doubled as the US championships.
Trammell cruised to victory in 7.41 seconds in the 60 meters hurdles, while Olympic bronze medallist David Oliver was second in 7.54 seconds.
Dunn unexpectedly won the women's 400 meters in a meet record 50.86 seconds, another world best for 2010.
Hurdler Bershawn Jackson won the men's 400 meters title in 45.41 seconds, while Reese leapt 6.89m to win the long jump.
Defending world indoor gold medallist Lolo Jones was edged by Ginnie Powell in the women's 60 meters hurdles, but still made the team. Powell clocked 7.87 seconds with Jones second in 7.89.
Christian Cantwell tuned up for a world title defense in the men's shot put with a winning heave of 21.13 meters.
In Melbourne, Kenya's Olympic 1500m champion Asbel Kiprop will this week headline an international contingent of athletes at the IAAF Melbourne Track Classic, organizers said Monday.
Kiprop, 20, who was confirmed as the 2008 Olympic gold medallist after Bahrain's Rashid Ramzi was stripped of his Beijing title for doping, will feature in Thursday's strong 1500m field.
Joining Kiprop will be reigning Commonwealth champion and Olympic silver medallist Nick Willis of New Zealand, fellow Kenyan Collins Chemboi and Australian champion Jeff Riseley.
Kiprop, who has a personal best time of 3:31.20 over 1500m, will reprise his rivalry with Beijing Olympics runner-up Willis.
Kiprop boosts an overseas group for the IAAF-sanctioned track classic which includes dual Olympic 400m medallist David Neville of the United States, Kenyan 800m runner David Rudisha, Finland's former world javelin champion Tero Pitkamaki and world and Olympic shot put champion Valerie Vili of New Zealand.
Australia's world and Olympic pole vault champion Steve Hooker, world discus champion Dani Samuels, world championships long jump bronze medallist Mitchell Watt and Beijing Olympic 100m hurdles silver medallist Sally McLellan are also competing.
